Effects of dedifferentiated fat cells on neurogenic differentiation and
cell proliferation in neuroblastoma cells
Background: Recent reports have demonstrated the ability of mesenchymal
stem cells (MSCs) to induce differentiation of neuroblastoma (NB) cells.
Properties of dedifferentiated fat cells (DFAT) are similar to those of
MSCs. In this study, we investigated if DFAT can induce NB cell
differentiation and suppress cell proliferation. Procedure: DFAT was
obtained from mature adipocytes isolated from adipose tissue from a
ceiling culture. NB cells were cultured in a medium with or without
DFAT. Subsequently, these cells were cultured in a DFAT-conditioned
medium (CM) with or without phosphatidylinositol 3 kinase (PI3K)
inhibitor. The neurites lengths were measured, and the mRNA expression
levels of the neurofilament (NF) and tubulin beta III (TUBβ3) were
assessed using quantitative real-time RT-PCR. Cell viability was
assessed by the WST-1 assay.