loading page

MicroRNA319-targeted RaTCP1 Regulates Heterophyllic Alteration in North American Lake Cress
  • +6
  • Shiqi Hu,
  • Gaojie Li,
  • Jingjing Yang,
  • Tomoaki Sakamoto,
  • Shuka Ikematsu,
  • Xuyao Zhao,
  • Seisuke Kimura,
  • Elizabeth Schultz,
  • Hongwei Hou
Shiqi Hu
Institute of Hydrobiology Chinese Academy of Sciences
Author Profile
Gaojie Li
Institute of Hydrobiology Chinese Academy of Sciences
Author Profile
Jingjing Yang
Institute of Hydrobiology Chinese Academy of Sciences
Author Profile
Tomoaki Sakamoto
Kyoto Sangyo University
Author Profile
Shuka Ikematsu
Kyoto Sangyo University
Author Profile
Xuyao Zhao
Institute of Hydrobiology Chinese Academy of Sciences
Author Profile
Seisuke Kimura
Kyoto Sangyo University
Author Profile
Elizabeth Schultz
University of Lethbridge Faculty of Arts and Science
Author Profile
Hongwei Hou
Institute of Hydrobiology Chinese Academy of Sciences

Corresponding Author:[email protected]

Author Profile

Abstract

Heterophylly, the plasticity of leaf form in response to environmental conditions, occurs in aquatic and amphibious plants where it modulates water availability, gas exchange and photosynthesis to maximize plant productivity. Rorippa aquatica produces simple leaves in terrestrial conditions but dissected leaves under submerged conditions. Regulation of CIN-TCP transcription factors by miR319 controls leaf complexity in a number of species and we provide evidence that this regulatory module acts in R. aquatica heterophylly. RaTCP1 was identified as the most likely target of Raq-miR319b. Under conditions that induced increased leaf complexity, RaTCP1 expression was reduced whereas Raq-miR319b expression was increased. Overexpression of Raq-miR319 in Arabidopsis thaliana reduced TCP gene expression and increased leaf serration. Similarly, a repressive form of RaTCP1 expressed in either A. thaliana or R. aquatica resulted in more complex leaves. Our results indicate that the environmental induction of dissected leaves in R. aquatica occurs through down-regulation of RaTCP by Raq-miR319.