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PTPRC overexpression predicts a poor prognosis in pediatric acute myeloid leukemia and is correlated with immune cell infiltration
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  • Gaili Guo,
  • Qianpeng Li,
  • Chunpu Li,
  • Ping Li,
  • Dongmei Guo
Gaili Guo
New Century International Children’s Hospital
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Qianpeng Li
Weifang People's Hospital
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Chunpu Li
Taian City Central Hospital
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Ping Li
Dezhou People's Hospital
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Dongmei Guo
Taian City Central Hospital

Corresponding Author:[email protected]

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Abstract

Background:Acute myeloid leukemia (AML) is a molecularly heterogeneous disease that accounts for approximately 25% of childhood leukemia cases. In this study, we purposed to identify survival-associated genes in pediatric AML patients and investigate potential immunotherapy targets. Methods: After retrieving and processing the data from Gene Expression Omnibus (GEO) web resource, we determined hub genes in AML by the differentially expressed genes (DEGs) assessment and the weighted correlation network analysis (WGCNA). The prognostic significance of the hub gene was further evaluated with the Kaplan-Meier survival based on TARGET dataset. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses of DEGs were performed. Then, via the CIBERSORT algorithm approach, we investigate the association between the key gene and the infiltration levels of tumor-infiltrating immune cells. Results: High PTPRC expression was linked to a worse overall survival rate (p<0.001) in 287 pediatric AML patients. Risk subgroup analysis showed similar results in low and high risk group, respectively (p=0.007; p=0.013). Meanwhile, multivariate analysis indicated that high PTPRC expression was an independent favorable prognostic factor for overall survival (p=0.04, HR=1.24, 95% CI=1.01-1.5). Moreover, the immune infiltration assessment results demonstrated that the expression of PTPRC level was significantly correlated with invading levels of activated dendritic cells (p<0.001). Conclusion: Overexpression of PTPRC indicates poor prognosis, and its expression level is correlated with invading levels of activated dendritic cells. PTPRC could be a promising immunotherapy target for pediatric AML.