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Down-regulation of CD73 on CD4 T Cells from CHIKV Chronic Patients
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  • PEDRO PALMEIRA,
  • Bruna Gois,
  • Isabel Guerra-Gomes,
  • Rephany Peixoto,
  • Cínthia Dias,
  • Josélio Araújo,
  • Ian Amaral,
  • Tatjana Keesen
PEDRO PALMEIRA
UFPB
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Bruna Gois
UFPB
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Isabel Guerra-Gomes
UFPB
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Rephany Peixoto
UFPB
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Cínthia Dias
UFPB
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Josélio Araújo
Universidade Federal do Rio Grande do Norte
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Ian Amaral
UFPB
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Tatjana Keesen
UFPB

Corresponding Author:[email protected]

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Abstract

Chikungunya is an important mosquito-borne disease caused by the arthritogenic Chikungunya virus (CHIKV) and is responsible for sporadic outbreaks all around the world. CD4+ T cells seem to have an important role in the pathogenesis of Chikungunya disease. Nevertheless, the mechanisms by which this occurs are not yet fully elucidated. CD39 and CD73 are ectoenzymes involved in the hydrolysis of pro inflammatory extracellular ATP and generation of immunosuppressive adenosine. The expression of these ectoenzymes seems to be modulated in some arthritogenic diseases. Thus, we investigated peripheral CD4+ T cells from acute and chronic chikungunya patients concerning ectonucleotidases CD39 and CD73 expression. Our group also assessed cytokine and cytolytic granules production and coinhibitory receptors expression in both phases of the disease using flow cytometry. In the acute phase, patients with chronic Chikungunya displayed increased levels of PD-1 and CTLA-4 when compared to healthy individuals. Moreover, there was higher production of IL-10 and IFN-γ in acute phase compared to healthy control and chronic groups. Besides, while no changes were observed in granzyme B expression, during chronic phase perforin production was diminished. Finally, no difference in CD39 expression was found in both phases of the disease. However, the ectoenzyme CD73 decreased during the chronic phase, suggesting a possible modulation of extracellular adenosine by CD4 T cells that may be involved in the persistence of arthritogenic symptoms. Thus, our data provide new insights into the characteristics of CD4+T cells highlighting molecules that may be associated with the pro-inflammatory hallmark of this disease.