loading page

MiR-193a-3p targets LGR4 to promote the inflammatory response in endometritis
  • +4
  • Baoyi Yin,
  • talha umar,
  • Xiaofei Ma,
  • Yu Chen,
  • Nuoer Chen,
  • Zhimin Wu,
  • Ganzhen Deng
Baoyi Yin
Huazhong Agricultural University
Author Profile
talha umar
Author Profile
Xiaofei Ma
Author Profile
Nuoer Chen
Author Profile
Ganzhen Deng

Corresponding Author:[email protected]

Author Profile

Abstract

Solving the reproductive barriers of dairy cows has become one of the most critical factors determining the dairy industry’s development. Clinically, inflammation disease like endometritis is one of the leading causes in reducing dairy production’s financial viability. MiR-193 family can induce cells apoptosis and differentiation has been reported in various diseases. LGR4 plays a vital role in reproductive system development and immune system regulation, and it is closely related to animal reproductive function and cytokine regulation. In this study, we observed a negative relationship between miR-193a-3p and LGR4 expression level in both inflammatory tissues and cells, and the expression level of miR-193a-3p and LGR4 in bovine endometrial epithelial cells (BENDs) is regulated by lipopolysaccharide (LPS) stimulation time and dose-dependent. Subsequently, miR-193a-3p mimics and inhibitors were used to explore its functions in the inflammation response process, finding that overexpression of miR-193a-3p markedly increase the expression level of pro-inflammatory cytokines induced by LPS, such as IL-1β, IL-6 and TNF-α, while the group in which transfected inhibitor is on the contrary. Of note, immunofluorescence and western blotting results showed that miR-193a-3p enhances LPS-induced NF-κB p65 phosphorylation through targeting LGR4, whereas inhibiting miR-193a-3p could suppress the activation of NF-κB pathway significantly. In conclusion, our study firstly reported the mechanism by which miR-193a-3p targets LGR4 to elevate the inflammatory response in bovine endometrium injury, thereby implying that knockdown miR-193a-3p may lay the theoretical and practical basis for drug development of alleviating endometritis in dairy cows.