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First detection and genetic characterization of a novel kirkovirus in diarrhoeal piglets in China
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  • Panpan Tong,
  • Meiling Ren,
  • Zunba Wang,
  • Xiaozhen Song,
  • Lei Zhang,
  • Guangwei Song,
  • Chenyang Jia,
  • Ruli Duan,
  • Nuerlan Palidan,
  • Ling Kuang,
  • Jinxin Xie
Panpan Tong
Xinjiang Agricultural University
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Meiling Ren
Xinjiang Agricultural University
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Zunba Wang
Tecon Bio-technology Co,. LTD
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Xiaozhen Song
Xinjiang Agricultural University
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Lei Zhang
Xinjiang Agricultural University
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Guangwei Song
Tecon Bio-technology Co,. LTD
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Chenyang Jia
Xinjiang Agricultural University
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Ruli Duan
Xinjiang Agricultural University
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Nuerlan Palidan
Xinjiang Agricultural University
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Ling Kuang
Xinjiang Agricultural University
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Jinxin Xie
Xinjiang Agricultural University

Corresponding Author:[email protected]

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Abstract

In this study, a novel kirkovirus (kirV) was detected in anal swabs of piglets with diarrhoea, the positive rate was 100% (149/149), but this virus was detected in one of 261 clinically healthy piglets, this result suggests a strong relationship between the kirV and the diarrhoeal disease. We obtained the whole-genome sequences of three kirVs (named Cj-D5, Cj-D32 and Cj-D43), with the length of 3750 nt, shared 86.6%–89.0% nt-identity with equine kirV Equ1 and kirV Cj-7-7. Furthermore, three viruses share32.1%–93.4%, and 22.2%–27.1% of the Rep protein sequence identity with available reference strains of the proposed family Kirkoviridae, and the family Circoviridae, respectively. Moreover, like kirV Equ1 and Cj-7-7, RCR domain and P-loop NTPase domains of Rep protein, and nonanucleotide motif (CAATATTAC), of three viruses, were similar to Circoviruses and Cycloviruses. Phylogenetic analysis showed that these viruses were grouped together with members in the proposed family Kirkoviridae. This is the first report that kirV circulates in piglets with diarrhoea, and next studies are needed to determine the pathogenesis of this virus.