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Expression and significance of ELANE and CTSG in primary acquired nasolacrimal duct obstruction
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  • Xinyue Yu,
  • Kerui Wang,
  • Jing Li,
  • Yu Hu,
  • Ziwei Meng,
  • Jingshu Zeng,
  • Han Du,
  • Chaojuan Wen,
  • Meiqing Chen,
  • Shuiling Li,
  • Yongqing Shen,
  • Rongxin Chen,
  • Huangxuan Shen,
  • Xuanwei Liang
Xinyue Yu
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Kerui Wang
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Jing Li
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Yu Hu
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Ziwei Meng
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Jingshu Zeng
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Han Du
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Chaojuan Wen
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Meiqing Chen
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Shuiling Li
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Yongqing Shen
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Rongxin Chen
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Huangxuan Shen
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology
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Xuanwei Liang
Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology

Corresponding Author:[email protected]

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Abstract

Purpose: To clarify the different proteins between primary acquired nasolacrimal duct obstruction (PANDO) patients and healthy people and to explore the underlying pathogenesis. Methods: We collected tears from research subjects and used proteomics methods to determine protein expression.The target differential proteins were screened by bioinformatics analysis and and provide proof of histological staining Results: The proteomics experiments included 4 patients and 4 controls, all female. A total of 369 differential proteins (DEPs) were identified between two groups (FC > 1.5, P value < 0.05). The DEPs are mainly involved in the immune response and inflammatory processes, included ELANE and CTSG. Immunohistochemistry experiments found the positive expression of ELANE and CTSG in the PANDO nasolacrimal duct, but expression was not related to the degree of pathological injury (P=0.929; P=0.779). ELANE and CTSG was significantly higher in chronic dacryocystitis than in acute (P<0.001; P=0.031; P=0.031). There was a significant positive correlation between ELANE and CTSG expression. Conclusions: PANDO patients have tons of DEPs in tears compared to healthy people, which are mainly involved in inflammatory and immune processes. The positive expression of ELANE and CTSG and are strongly associated with a chronic inflammatory process of PANDO.