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Dandelion detoxification lotion promotes the healing of venous ulcers by inhibiting ferroptosis via the CoQ-FSP1 axis
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  • Yongpan Lu,
  • De jie Zhao,
  • Ming Liu ,
  • Guoqi Cao,
  • Chunyan Liu,
  • Siyuan Yin,
  • Ru Song,
  • Jiaxu Ma,
  • Rui Sun,
  • Zhenjie Wu,
  • Jian Liu,
  • Yibing Wang
Yongpan Lu
Shandong University of Traditional Chinese Medicine
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De jie Zhao
Shandong University of Traditional Chinese Medicine Affiliated Hospital
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Ming Liu
Affiliated Hospital of Shandong University of Traditional Chinese Medicine
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Guoqi Cao
Shandong University
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Chunyan Liu
The First Affiliated Hospital of Shandong First Medical University
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Siyuan Yin
The First Affiliated Hospital of Shandong First Medical University
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Ru Song
The First Affiliated Hospital of Shandong First Medical University
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Jiaxu Ma
Shandong University
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Rui Sun
Shandong University
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Zhenjie Wu
Shandong University
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Jian Liu
The First Affiliated Hospital of Shandong First Medical University
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Yibing Wang
The First Affiliated Hospital of Shandong First Medical University

Corresponding Author:[email protected]

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Abstract

Objective: Dandelion detoxification lotion (DDL, number Z01080175) clears heat, detoxifies, activates the blood circulation, reduces swelling, and dispels decay and pus. The aim of this study was to investigate the mechanism of action by which DDL functions in the treatment of venous ulcers (VUs). Methods: Normal tissues as well as VU tissues before and after DDL treatment were collected from nine VU patients in the hospital with ethical approval. These three tissues were subjected to Prussian blue iron staining, immunoblotting, immunohistochemistry, immunofluorescence, and qPCR to detect the expression of ferroptosis suppressor protein 1 (FSP1), coenzyme Q (CoQ) 4-hydroxynonenal (4-HNE), and glutathione peroxidase 4 (GPX4). After successful validation of the heme-induced human foreskin fibroblast (HFF) ferroptosis model, lyophilized DDL powder was added to the cells, and the cells were subjected to viability assays, immunoblotting, flow cytometry, glutathione (GSH) and malonaldehyde (MDA) assays, electron microscopy and qPCR assays. Results: Ferroptosis in VU tissues was stronger than that in normal tissues, and ferroptosis in VU tissues after DDL treatment was weaker than that before treatment. Inhibition of CoQ and FSP1 and transfection of FSP1 influenced the effects of DDL. Conclusions: Our results suggest that DDL may promote healing by attenuating ferroptosis in VUs and that DDL may promote VU healing by modulating the CoQ-FSP1 axis.