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Identifying Bacterial and Eukaryotic Communities Related to Marine Mucilage with eDNA Metabarcoding
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  • Işılay Çelik,
  • Berkay Gülen,
  • Pelin Taş,
  • Gizem Geçgil,
  • E. Keskin
Işılay Çelik
Ankara University
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Berkay Gülen
Ankara University
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Pelin Taş
Ankara University
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Gizem Geçgil
Ankara University
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E. Keskin
Ankara University

Corresponding Author:[email protected]

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Abstract

Marine mucilage is a cluster of visible organic compounds with a viscous structure and can be found in marine water. Mucilage has been previously reported in the Adriatic Sea, the Tyrrhenian Sea, the Mediterranean Sea, and the Ionian Sea. Identification of the mucilage composition is essential to determine which microbial communities are abundant and examine potential marine diseases that affect almost all organisms. For this purpose, eDNA metabarcoding offers quick, reliable, and diverse advantages. In this study, mucilage samples taken from Bandırma Bay are subjected to eDNA metabarcoding by using 16S rRNA and 18S rRNA regions (V3 and V4, respectively) to identify bacterial and eukaryotic diversity. ObiTools and SILVAngs were used for the bioinformatic analysis, and all species-level identifications were made using the NCBI GenBank database. According to the results, abundant species belonged to Polaribacter, Fusibacter, Vibrio, Formosa, Ulvibacter, and Marinifilum in bacterial communities, while species from Alexandrium, Gyrodinium, Thalassiosira, Eucarida, and Protodinium were found as eukaryotic communities populated the mucilage samples. This is the first marine mucilage metabarcoding study evaluating both bacterial and eukaryotic communities.
21 Aug 2023Submitted to Marine Ecology
21 Aug 2023Assigned to Editor
21 Aug 2023Submission Checks Completed
30 Aug 2023Reviewer(s) Assigned