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MiR-21 regulates proliferation and apoptosis of mesangial cells via PTEN/PI3K/AKT pathway in lupus nephritis
  • Zhifeng Gu,
  • Qian He,
  • Juan Ji
Zhifeng Gu
Affiliated Hospital of Nantong University

Corresponding Author:[email protected]

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Qian He
Affiliated Hospital of Nantong University
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Juan Ji
Affiliated Hospital of Nantong University
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Abstract

Objective: Systemic lupus erythematosus(SLE)is an autoimmune disease involving multiple organs and systems throughout the body. The pathogenesis is complex and has not been fully elucidated. Excessive proliferation of human renal mesangial cells(HRMC)and endothelial cells leads to the deposition of massive immune complexes and the formation of crescents, which is the main cause of lupus nephritis (LN) and one of the main complications of SLE. Recent studies have demonstrated that miR-21 plays an important role in the progression of SLE, but the mechanism remains unclear. The purpose of this study was to determine the expression of miR-21 in renal tissues of MRL/lpr mice and the effect of miR-21 on the proliferation and apoptosis of HRMC and its mechanism. Methods: Real-time fluorescent quantitative PCR (qRT-PCR) was used to detect miR-21 in kidney tissues of C57BL/6 and MRL/lpr mice. Flow cytometry was used to detect the apoptosis of cells. Western blot was used to detect the expression of BAX, BCL-2 and PTEN/ PI3K/AKT signaling pathway. Results: qRT-PCR results showed that miR-21 was significantly upregulated in MRL/lpr mice. Western blot showed that miR-21 affected apoptosis of HRMC by BAX and BCL-2 and participated in cell proliferation through the PTEN/ PI3K/AKT signaling pathway. Conclusion: MiR-21 regulates mesangial cells proliferation and apoptosis in LN via PTEN/ PI3K/ AKT signaling. These results reveal the molecular basis of the influence of miR-21 on renal mesangial cells and provide a new approach for cell therapy of lupus nephritis.