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Stomatal characteristics and their Position relative to leaf epidermal cells in ten Maple species of hyrcanian forests
  • Farzaneh Moghbel esfahani ,
  • Vahide Payamnoor,
  • Ali sattarian
Farzaneh Moghbel esfahani
Gorgan University of Agricultural Sciences and Natural Resources
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Vahide Payamnoor
Gorgan University of Agricultural Sciences and Natural Resources

Corresponding Author:[email protected]

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Ali sattarian
Gonbad Kavous University
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Abstract

In the present study, we examined the structure of stomata in 7 native species of hyrcanian forests (Acer hyrcanum, A.velutinum Boiss., A.campestre, A.platanoides L., A.cappadocicum, A.monspessulanum, A.amazandaranicum) plus non-native species that fully adapted (A.negundo, A.negundo variegatum, and A.palmatum). Light and electron microscopy were used to determine the form and position of the stomata in relation to the leaf epidermal cells. The length, width, shape, area, perimeter, and stomatal density were all measured. It was found that the stomata type of A.negundo varengiayum, A.campestre, A.hyrcanum, A.mazandaranicum, A.monsspesulanum is anomocytic, in A.platanoides and A.cappadocicum anomocytic with wavy subsidiary cells, anisocytic in A.palmatum, parasitic in A.velutinum, and actinocytic A.negundo. In terms of the location of stomata relative to adjacent epidermal cells, we identified three types. In the first type, the stomata were flush with adjacent epidermal cells (A.cappadocicum, A.negundo, A.platanoides), in the second type, higher (A.negundo variegatum) and the third type, lower (A.velutinum, A.monspesulanom, A.campestre, A. mazandaranicum, A.hyrcanum). The principal component analysis was used to determine the essential stomatal traits in differentiating between species. We investigated tree-stemmed distribution in the coordinate axis space based on two main components and performed cluster analysis based on the stoma characteristics. A.platanoides, A.negundo, A.negundo variegatum were in one cluster, and the other species were in separate clusters. The calculation of dissimilarity among the studied species indicates the slightest similarity between A.negundo and A.hyrcanum and the highest similarity between A.campestre and A.mazandaranicum. The discriminant analysis results identified stomatal density as the essential factor in the differentiation between the studied species