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Interleukin-37 suppresses oxidized-LDL induced pro-osteogenic response by downregulating NF-κB and PERK-eIF2alpha-ATF4 pathways in human coronary artery endothelial cells
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  • Jilin Li,
  • Changyi Zhang,
  • Kaijian Hou,
  • Xianfeng Yan,
  • Xiaojun Huang,
  • Liemin Hu,
  • Xiangna Cai
Jilin Li
Shantou University Medical College
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Changyi Zhang
Shantou University Medical College
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Kaijian Hou
The First Affiliated Hospital of Shantou University Medical College
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Xianfeng Yan
The First Affiliated Hospital of Shantou University Medical College
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Xiaojun Huang
Shantou University Medical College
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Liemin Hu
People's Hospital of Ruyuan Yao Autonomous County
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Xiangna Cai
The First Affiliated Hospital of Shantou University Medical College

Corresponding Author:[email protected]

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Abstract

OBJECTIVE: Coronary atherosclerosis is an age-related disease that originates from osteogenic differentiation of coronary arterial endothelial cells (CAECs) induced by intimal injury. CAECs express excess levels of osteogenic factors in response to oxidative stress. This study aims to investigate the effect of IL-37 on high cellular level of oxidized low-density lipoprotein (ox-LDL) -induced CAECs and its potential mechanism. MATERIALS AND METHODS: Cultured CAECs were randomly divided into two groups: control group and IL-37 pretreatment group. Cells were cultured with ox-LDL (0, 40, or 80μg/ml) for 0h,1h,2h,3h, respectively. Western blot analysis and real-time PCR were performed to assess the associated gene and protein expression, respectively.ATF-4 and Phosphorylated eIF2α were identified by immunofluorescence staining. RESULTS: Treatment of HCAECs with recombinant human IL-37 inhibits BMP-2 and ALP protein expression and down-regulates phosphorylated-NF-κB p65 expression(p<0.05). In vitro, HCAECs pretreated with recombinant human IL-37 exhibit significantly low level of phosphorylated PERK and eIF2α and diminished AFT-4 expression compared to control group (p<0.05). CONCLUSIONS: These findings suggest that IL-37 suppresses pro-osteogenic responses induced by ox-LDL that result in down-regulation of NF-κB and PERK-eIF2α-ATF4 pathways in HCAECs. Since these pro-osteogenic factors are involved in smooth muscle cell calcification. Our study indicates IL-37 could have therapeutic potential for the treatment of progressive coronary artery calcification (CAC).
07 Apr 2023Submitted to Immunity, Inflammation and Disease
11 Apr 2023Submission Checks Completed
11 Apr 2023Assigned to Editor
11 Apr 2023Review(s) Completed, Editorial Evaluation Pending
15 Apr 2023Reviewer(s) Assigned
17 Jul 2023Editorial Decision: Revise Major
17 Aug 20231st Revision Received
18 Aug 2023Submission Checks Completed
18 Aug 2023Assigned to Editor
18 Aug 2023Review(s) Completed, Editorial Evaluation Pending
19 Aug 2023Reviewer(s) Assigned
31 Aug 2023Editorial Decision: Revise Major
15 Oct 20232nd Revision Received
13 Nov 2023Submission Checks Completed
13 Nov 2023Assigned to Editor
13 Nov 2023Review(s) Completed, Editorial Evaluation Pending
16 Nov 2023Reviewer(s) Assigned