The term amyloidoma applies to localized deposits of amyloid in the
absence of systemic amyloidosis. Skeletal and soft tissue amyloidomas
are very rare and the pathogenesis is usually associated with
lymphoproliferative disorders or because of local chronic inflammation.
Due to the rarity of the disease and the limited application of
proteomics for diagnostic purposes, it is difficult to find datasets of
mass spectrometry-based characterization of amyloidomas. Here we report
the histological and immunohistochemical features of four cases of
musculoskeletal amyloidoma in association with laser capture
microdissection (LCM) and bottom-up microproteomics. Proteomics
techniques allowed to elucidate the nature of the amyloid protein
deposit, improving the results obtained by immunohistochemistry (IHC).
IHC results were confirmed in two cases while laser-capture
microdissection coupled with bottom-up microproteomics was necessary to
type the other two cases, for which IHC was inconclusive.