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Proteomics and bioinformatics investigations to improve serological diagnosis of canine brucellosis
  • +6
  • Mirella Luciani,
  • Ivanka Krasteva,
  • Tiziana Di Febo,
  • Fabrizia Perletta,
  • Federica D'Onofrio,
  • Fabrizio De Massis,
  • Nicola D'Alterio,
  • Flavio Sacchini,
  • Manuela Tittarelli
Mirella Luciani
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G Caporale
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Ivanka Krasteva
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G Caporale
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Tiziana Di Febo
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G Caporale

Corresponding Author:[email protected]

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Fabrizia Perletta
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G Caporale
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Federica D'Onofrio
University of Teramo Faculty of Biosciences and Agro-Food and Environmental Technologies
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Fabrizio De Massis
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G Caporale
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Nicola D'Alterio
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G Caporale
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Flavio Sacchini
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G Caporale
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Manuela Tittarelli
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G Caporale
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Abstract

Brucella canis is pathogenic for dogs and humans. Serological diagnosis is a cost-effective approach for disease surveillance, but a major drawback of current serological tests is the cross-reactivity with other bacteria that results in false positive reactions, and development of indirect tests with improved sensitivity and specificity remain a priority. A western blotting assay was developed to define the serum antibody patterns associated to infection using a panel of positive and negative dog sera. B. canis positive sera recognized immunogenic bands ranging from 7 to 30 kDa that were then submitted to ESI–LC-MS/MS and analyzed by bioinformatics tools. A total of 398 B. canis proteins were identified.. Bioinformatics tools identified 16 non cytoplasmic immunogenic proteins predicted as non-homologous with the most important Brucella cross-reactive bacteria and 9 B. canis proteins non-homologous to B. ovis; among the latter, one resulted non-homologous to B. melitensis. The western blotting test developed was able to distinguish between infected and non-infected animals and may serve as confirmatory test for the serological diagnosis of B. canis. The mass spectrometry and in silico results lead to the identification of specific candidate antigens that pave the way for the development of more accurate indirect diagnostic tests.
22 Dec 2022Submitted to Clinical Applications
23 Dec 2022Submission Checks Completed
23 Dec 2022Assigned to Editor
23 Dec 2022Review(s) Completed, Editorial Evaluation Pending
23 Dec 2022Reviewer(s) Assigned
04 Apr 2023Editorial Decision: Revise Major
26 May 20231st Revision Received
26 May 2023Review(s) Completed, Editorial Evaluation Pending
01 Jun 2023Reviewer(s) Assigned
16 Jun 2023Editorial Decision: Revise Minor
10 Jul 2023Review(s) Completed, Editorial Evaluation Pending
10 Jul 20232nd Revision Received
18 Jul 2023Editorial Decision: Accept