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Evaluation of Candida species-specific T cell immune response in human peripheral blood mononuclear cells
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  • Balaji Pathakumari,
  • Weida Liu,
  • Qiong Wang,
  • kong xue,
  • Santosh Chokkakula,
  • Venkatrao Nunna
Balaji Pathakumari
Chinese Academy of Medical Sciences Institute of Dermatology
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Weida Liu
Chinese Academy of Medical Sciences Institute of Dermatology

Corresponding Author:[email protected]

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Qiong Wang
Chinese Academy of Medical Sciences Institute of Dermatology
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kong xue
Chinese Academy of Medical Sciences Institute of Dermatology
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Santosh Chokkakula
Chungbuk National University College of Medicine
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Venkatrao Nunna
Washington University School of Medicine in Saint Louis
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Abstract

Non-albicans Candida (NAC) species are emerging causes of all Candidemia infections, but relatively less is known about the immune responses induced by these species. In this study, we compared the ability of cytokine production by human peripheral blood mononuclear cells (PBMC) upon stimulation with different Candida species. We measured secreted cytokines by ELISA and checked the functional profile of T cell responses at a single-cell level by multi-color flow cytometry technique. Though there was a differential expression of cytokines against the Candida species, a significant difference was observed in the levels of IFN-γ, TNF-α, IL-10, IL-12p40, and IL-23 (p<0.05) between the Candida species. There is a significant difference was observed between C. albicans and C. glabrata (p-0.026) in the levels of TNF-α. C. glabrata showed significant difference with C. albicans, C. parapsilosis and C. krusei in the levels of IL-10. Despite the percentages of CD4+ and CD8+ expressing Th1, Th2 and Th17 cytokines being higher in stimulated PBMC, none of the Candida species showed significant differences. The levels of secreted IL-17A and IL-23 were consistently lower in Candida species regardless of the stimulus used. Here we showed the differential regulation of the Th1, Th2 and Th17 during Candida species stimulation of the immune system in vitro. Our results also indicate that while C. albicans induces a Th1 response, C. glabrata induces Th2 cellular responses. This study could enhance our knowledge of differential immune responses induced by Candida species and may help to develop immunotherapeutics and vaccines.