loading page

Phylogenetic Analysis Detected Newly Identified Phylogroups in Uropathogenic Escherichia coli Isolates in Iraq
  • +5
  • Nima Yakhchalian,
  • Hiba J Hamza,
  • Huda H Al-Hasnawy,
  • Layth Jasim Mohammad,
  • Mohammed R Judi,
  • Hider M H Al-Shirifi,
  • Isaac Karimi,
  • Nima Yakhchalian
Nima Yakhchalian

Corresponding Author:[email protected]

Author Profile
Hiba J Hamza
Babylon Governorate, Department of Microbiology, College of Medicine, Babylon University
Huda H Al-Hasnawy
Babylon Governorate, Department of Microbiology, College of Medicine, Babylon University
Layth Jasim Mohammad
Babylon Governorate, Department of Pharmacology, College of Medicine, Babylon University
Mohammed R Judi
Babylon Governorate, Department of Microbiology, College of Medicine, Babylon University
Hider M H Al-Shirifi
Babylon Governorate, Department of Microbiology, College of Medicine, Babylon University
Isaac Karimi
Department of Biology, Faculty of Science, Laboratory for Computational Physiology, Razi University
Nima Yakhchalian
Department of Biology, Faculty of Science, Razi University

Abstract

Escherichia coli (E. coli) strains have been classified into eight distinct phylogenetic clusters as per a novel quadruplex PCR method. Nevertheless, the precise phylogenetic relationship among these bacterial lineages remains unclear. The Clermont phylotyping method has been utilized by the current study to further clarify E. coli phylogenetic clusters and assess resistance to antibiotics showed by uropathogenic E. coli (UPEC) strains in Iraq. Forty-two UPEC isolates were assessed for antibiotic sensitivity through a disk diffusion test, and the novel Clermont phylotyping method was utilized for the phylogenetic identification of isolates. The research findings revealed the varying prevalence of distinct phylogroups at the hospitals of Babylon province, Iraq, with Phylogroup B2, as the predominant group accounting for 47.61%, followed by Clade I (14.28%), B1 (11.90%), A (9.52%), D (4.76%), C (2.38%), and an unidentified phylogroup (9.52%). Additionally, out of the 42 Uropathogenic E. coli isolates studied, 37 (88.09%) showed multidrug resistance (MDR) and 5 isolates (11.90%) displayed extensive drug resistance (XDR). MDR and XDR strains within Phylogroup B2 accounted for 17 out of 37 cases (45.24%) and 3 out of a total of 5 cases (60%), respectively, indicating a large proportion of MDR and XDR UPEC isolates within phylogroup B2. Moreover, two new phylogroups, namely C and clade I, were identified, linked respectively to E. coli sensu stricto and cryptic E. coli. Thus, further studies are required to be conducted elsewhere to gain a better perception of both antibiotic-resistance characteristics and the occurrence of diverse phylogroups in Iraq.