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Evaluation Of The Potential Anticancer Activity Of Pyrazole-Acridine Derivative Synthesis In The SH-SY5Y Human Neuroblastoma Cell Line
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  • Yusuf KUCUKBAGRIACIK,
  • Muna ELMUSA,
  • Fatima ELMUSA,
  • Humeyra YILMAZ,
  • Mohammadreza DASTOURI
Yusuf KUCUKBAGRIACIK
Saglik Bilimleri Universitesi Gulhane Tip Fakultesi

Corresponding Author:[email protected]

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Muna ELMUSA
Elfurat Engineering Research and Development Limited Company
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Fatima ELMUSA
Eskisehir Teknik Universitesi
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Humeyra YILMAZ
Ankara Yildirim Beyazit Universitesi Tip Fakultesi
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Mohammadreza DASTOURI
Ankara Medipol Universitesi
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Abstract

Pyrazole is a nitrogen-containing five-membered heterocyclic compound, where nitrogen atoms are positioned at positions 1 and 2 within the ring. Acridine, a nitrogen-containing polycyclic aromatic compound. This study aimed to synthesize a novel pyrazole-acridine derivative, combining the structural elements of pyrazole and acridine into a single molecule, with the objective of enhancing its anticancer activity. The pyrazole-4-carbaldehyde derivative was cyclized with 5,5-dimethylcyclohexane-1,3-dione and 4-nitroaniline to synthesize the pyrazole-acridine derivative (3-ACH). Characterization of the compound FT-IR, NMR, HPLC-Q-Tof/MS and elemental analysis were used. In our investigation, we assessed the cytotoxic impact of 3-ACH on SH-SY5Y human neuroblastoma cells in a dose-dependent manner (50, 100, and 150 μg/mL) and in a time-dependent (12, 24, and 48hours) manner using the WST-1 test. The effects of 3-ACH on apoptosis were examined through immunostaining of BAX, Caspase-3, Caspase-8, Caspase-9 proteins. The results showed that the cytotoxicity of 3-ACH increased with doses and exposure times. Moreover, we observed a significant upregulation in the synthesis of BAX, Caspase-3,-8,-9 proteins. These findings suggest that 3-ACH has the potential to induce apoptosis in SH-SY5Y cells by activating both the intrinsic and extrinsic pathways, which may contribute to its cytotoxic effects. Our study supports 3-ACH as a promising anticancer agent.