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A sustainable strategy for biosynthesis of Rebaudioside D using a novel glycosyltransferase of Solanum tuberosum
  • Siyuan Ma,
  • Yuanyuan Ma
Siyuan Ma
Tianjin University
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Yuanyuan Ma
Tianjin University

Corresponding Author:[email protected]

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Bioconversion of Rebaudioside D faces high-cost obstacles. Herein, a novel glycosyltransferase StUGT converting Rebaudioside A to Rebaudioside D was screened and characterized, which exhibits stronger affinity and substrate specificity for Rebaudioside A than previously reported enzymes. A whole-cell catalytic system was thus developed using the StUGT strain. The production of Rebaudioside D was enhanced significantly by enhancing cell permeability, and the highest production of 6.12 g/L (yield=98.08%) by cell catalyst was obtained by statistical-based optimization. A new cascade process utilizing this recombinant strain and E. coli expressing sucrose synthase was further established to reduce cost through replacing expensive UDPG with sucrose. A StUGT-GsSUS1 system exhibited high catalytic capability, and 5.27 g/L Rebaudioside D was achieved finally without UDPG addition by systematic optimization. This is the best performance reported in cell-cascaded biosynthesis, which paves a new cost-effective strategy for sustainable synthesis of scarce premium sweeteners from biomass.
13 Nov 2023Submitted to Biotechnology Journal
13 Nov 2023Assigned to Editor
13 Nov 2023Submission Checks Completed
13 Nov 2023Review(s) Completed, Editorial Evaluation Pending
14 Nov 2023Reviewer(s) Assigned