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Chronic ethanol ingestion on the uterine horn of Calomys callosus
  • F. Martinez,
  • Marcelo Martinez,
  • D. Garbuio
F. Martinez
Universidade Estadual Paulista Julio de Mesquita Filho Departamento de Biologia estrutural e funcional

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Marcelo Martinez
Universidade Federal de Sao Carlos
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D. Garbuio
Universidade Federal de Sao Carlos
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Abstract

The present work was carried out to evaluate the possible effects of chronic alcohol intake on the histology and ultrastructure of the endometrial lining epithelium of the uterine horn of Calomys callosus. 20 adult female rodents, Calomys callosus, three months old, were used. These animals were divided equally into 2 experimental groups called control and alcoholic. The control group received a solid diet (mouse food) and tap water “ad libitum”. The alcoholic group received the same solid diet and ethanol was diluted to 20% in water. At the end of 120 days of treatment with 20% alcohol, all animals were anesthetized with ethyl ether and sacrificed. Daily observations of the animals’ behavior, carried out throughout the treatment period, made it possible to verify a certain lethargy of the animals in the alcoholic group, concerning the animals in the control group, regarding responses to physical and sound stimuli. Macroscopically, no difference was observed in the uterus between the groups analyzed. In light microscopy, the animals in the alcoholic group showed the same histological characteristics as the control group, however, a marked atrophy of the epithelial tissue covering the endometrium could be observed. This atrophy is confirmed by the results of transmission electron microscopy, where important changes were also observed in alcoholic animals: a large increase in lipid droplets, dispersed throughout the cytoplasm, digestive vacuoles, and an increase in the cisterns of the granular endoplasmic reticulum. The presence of invasive immune cells in the epithelial tissue was also observed. Scanning electron microscopy analyses showed the presence of lipid droplets in the endometrial lumen. Ethanol ingestion caused epithelial lesions in the uterine horn, causing apoptosis, and interfering with the secretory mechanism.