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Involvement of Escherichia coli YbeX/CorC in ribosomal metabolism
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  • İsmail Sarıgül,
  • Amata Žukova,
  • Emel Alparslan,
  • Sille Remm,
  • Margus Pihlak,
  • Niilo Kaldalu,
  • Tanel Tenson,
  • Ülo Maiväli
İsmail Sarıgül
Tartu Ulikooli Tehnoloogiainstituut

Corresponding Author:[email protected]

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Amata Žukova
Tartu Ulikooli Tehnoloogiainstituut
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Emel Alparslan
Tartu Ulikooli Tehnoloogiainstituut
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Sille Remm
Tartu Ulikooli Tehnoloogiainstituut
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Margus Pihlak
Tallinna Tehnikaulikool Kuberneetika instituut
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Niilo Kaldalu
Tartu Ulikooli Tehnoloogiainstituut
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Tanel Tenson
Tartu Ulikooli Tehnoloogiainstituut
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Ülo Maiväli
Tartu Ulikooli Tehnoloogiainstituut
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Abstract

YbeX of Escherichia coli, a member of the CorC protein family, is encoded in the same operon with ribosome-associated proteins YbeY and YbeZ. Here, we report the involvement of YbeX in ribosomal metabolism. The ΔybeX cells accumulate distinct 16S rRNA degradation intermediates in the 30S particles and the 70S ribosomes. E. coli lacking ybeX has a lengthened lag phase upon outgrowth from the stationary phase. This growth phenotype is heterogeneous at the individual cell level and especially prominent under low extracellular magnesium levels. The ΔybeX strain is sensitive to elevated growth temperatures and to several ribosome-targeting antibiotics that have in common the ability to induce the cold shock response in E. coli. Although generally milder, the phenotypes of the ΔybeX mutant overlap with those caused by ybeY deletion. A genetic screen revealed partial compensation of the ΔybeX growth phenotype by the overexpression of YbeY. These findings indicate an interconnectedness amongst the ybeZYX operon genes, highlighting their roles in ribosomal assembly and/or degradation.
27 Oct 2023Submitted to Molecular Microbiology
28 Oct 2023Assigned to Editor
28 Oct 2023Submission Checks Completed
28 Oct 2023Reviewer(s) Assigned
15 Nov 2023Review(s) Completed, Editorial Evaluation Pending
15 Nov 2023Editorial Decision: Revise Minor