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Phylogenetic Analysis and Genetic Diversity of Bacteria Strains isolated from oral cavity of Malnourished Children: Insights from Comparative Genomics
  • Abiodun Osatogbe
Abiodun Osatogbe
Kebbi State University of Science and Technology

Corresponding Author:[email protected]

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Abstract

Oral bacteria of malnourished children were investigated from Sokoto State Specialist Hospital. Insufficient nutrients create malnutrition, and food influences health. In microbiological isolation, aseptic mouth samples are evaluated. Swabs are spun across the pharynx for 15–20 seconds to collect samples. Self-sealing polythene containers convey samples to the lab for reliable analysis. Nutrient-sorbitol-bouillon agar isolate. Gram staining and biochemical testing identify healthy agar microorganisms. Dry slide examined using an oil-immersed objective lens. Characterising patient oral bacteria isolates using PCR. Some bacteria that were found in the patient’s mouth were Staph aureus, Escherichia coli, Streptococcus pyogenes, Cronobacter condiment, Photorhabdus luminscenes, Klebsiella aeruginosa, Bacillus tequillensis, and Yersinia molderath. 25% of isolates include Cronobacter condimenti. Klebsiella aeroginosa, 12.5% Staphylococcus aureus, 9.1% Escherichia coli, 9.1% Streptococcus pyogenes, 4.5% Bacillus tequillenses, and 4.5% Yersinia molderath were found in oral 15% of patients had Klebsiella aeroginosa, 10% Photorhabdus luminscenes, 10% Haemophilus influenza, 10% Staphylococcus aureus, E. coli, Streptococcus pyogenes, Bacillus megaterium, Cronobacter condimenti, and Bacillus tequillensis, and 10% each of We identified ten bacterial strains using colony morphology, biochemical assays, 16S rDNA sequence analysis, species-specific PCR, and phylogenetic trees of six isolated bacteria. A 1450-bp 16S RRNA gene amplicon separates PCR-amplified genomic DNA on agarose gel electrophoresis for all isolated bacteria. All isolated bacteria have 97.9–99.9% coverage and similarity. Sequence submission to NCBI and accession number assignment. Malnutrition affects millions of poor children worldwide. It can temporarily impede cognition, economic output, reproduction, disease, and death. Malnutrition prevention requires food security, healthcare, oral hygiene, and nutrition.