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Identification of environment-insensitive genes for oil content by a combination of transcriptome and genome-wide association analysis in Brassica napus
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  • Min Yao,
  • Dan He,
  • Wen Li,
  • Xinghua Xiong,
  • Xin He,
  • Zhongsong Liu,
  • Chunyun Guan,
  • Lunwen Qian
Min Yao
Hunan Agricultural University
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Xinghua Xiong
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Zhongsong Liu
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Chunyun Guan
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Lunwen Qian
Hunan Agricultural University

Corresponding Author:[email protected]

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Abstract

Seed oil content is an important agricultural trait in rapeseed breeding, which is controlled by multiple genes and influenced by environmental factors. In this study, we used transcriptome data from inbred lines with higher and lower oil contents at 35 days after pollination (DAP) to investigate environment-insensitive genes that exhibiting stable expression across three different environments. Consequently, a total of 405 environment-insensitive differentially expressed genes (DEGs) were identified, including 25 involved in lipid/fatty acid metabolism and 14 transcription factors. Among these genes, BnBZIP10-A09, BnaMYB61-A06, BnAPA1-A08, BnPAS2-A10, BnLCAT3-C05 and BnKASIII-C09 were located in previous reported confidence intervals and also found to exhibit significant associations with oil content in multiple different environments by GWAS of 50 re-sequenced Chinese semi-winter rapeseed accessions. Otherwise, we revealed the presence of additive effects among BnBZIP10-A09, BnKASIII-C09, and BnAPA1-A08, resulting in a significant increase in seed oil content. Meanwhile, co-expression network analysis revealed that most of these environment-insensitive DEGs are interconnected either directly or indirectly, thereby forming a molecular network implicated in the potential regulation of seed oil accumulation and stability. These results are important for us to further improve oil content accumulation and stability in rapeseed.