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Quantification of Subsurface Au nanoparticles in tissue simulating media using spatial frequency domain imaging
  • A. F. G. Monte,
  • A. C. M. Mendes,
  • Rolf Saager
A. F. G. Monte
Universidade Federal de Uberlandia

Corresponding Author:[email protected]

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A. C. M. Mendes
Universidade Federal de Uberlandia
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Rolf Saager
Universitetssjukehuset i Linkoping Rehabiliteringsmedicinska kliniken
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Abstract

Au nanoparticles (NPs) can be used in a wide range of applications, particularly in the biomedical area, where their strong absorption cross-section can enhance contrast in tagged biological tissues for tumor imaging and diagnostics. We propose a new methodology to measure the Au NP spatial distribution within superficial tissue volumes. Silicone tissue phantoms were used as a background medium with a priori knowledge of tissues’ optical properties measured by spatial frequency domain imaging (SFDI). A lookup-table (LUT) was then created for deducing the optical properties of embedded Au NPs with different spatial concentrations and depths at multiple spatial frequencies. We have developed an analytical solution from this LUT to determine the embedded NP concentration in-depth as a function of the measured spatial frequency-dependent optical absorption. SFDI was able to localize the NPs with a 3D perspective spatially. These results provide the groundwork for future studies on mapping NPs in-vivo.