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Bioinformatics comparison of hemolysin in different bacteria and experimental evaluation of anti-cancer properties
  • amir hossein kavianpour,
  • mandana Behbahani,
  • hassan mohabbatkar
amir hossein kavianpour
University of Isfahan

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mandana Behbahani
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hassan mohabbatkar
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Abstract

Cancer is one of the main causes of death in the world. Resistance to anticancer treatments in patients with advanced solid tumors leads to new treatments. Therefore, more alternative anticancer methods have been found over time with greater specificity against tumor cells and with less or no adverse effects on normal cells. In the microbial environment, pathogens such as Staphylococcus aureus, Bacillus cereus, or Streptococcus pyogenes produce hemolysin. This protein is used as an anti-cancer protein. To identify the production of hemolysin by bacteria, which can destroy cancer cells more effectively, different bacterial strains were first cultured in blood agar culture medium. The strains capable of complete hemolysis of red blood cells created a transparent zone in this environment and were selected for the next stages of the research. Then, to find out which strains have more ability to lyse red blood cells, the qualitative method of halo diameter measurement was used. Also, using quantitative methods, hemolysin strength in microtubes was determined compared to control samples. The results of the hemolysis in the microtube and the good test results showed similar results. knows Then, the cell viability test was performed with the partially purified proteins. Then, bioinformatics studies such as secondary structure investigation, physicochemical properties, pseudo amino acid composition, and molecular docking were performed.