loading page

Role of LIN28B in the regulation of ribosomal biogenesis and lipid metabolism in medulloblastoma brain cancer cells
  • +2
  • Ahmed Maklad,
  • Mohammed Sedeeq,
  • Richard Wilson,
  • Nuri Gueven,
  • Iman Azimi
Ahmed Maklad
University of Tasmania College of Health and Medicine
Author Profile
Mohammed Sedeeq
University of Tasmania College of Health and Medicine
Author Profile
Richard Wilson
University of Tasmania
Author Profile
Nuri Gueven
University of Tasmania School of Pharmacy and Pharmacology
Author Profile
Iman Azimi
Monash University Monash Biomedicine Discovery Institute

Corresponding Author:[email protected]

Author Profile

Abstract

Medulloblastoma (MB) is the most malignant paediatric brain cancer and current curative rates have not significantly improved recently. New diagnostic and prognostic biomarkers and improved treatment methods are needed to enhance MB patient outcomes. Our previous study showed that LIN28B, an RNA-binding protein, is a potential diagnostic and prognostic marker for MB, as well as a pharmacological target to inhibit the proliferation and stemness of MB cells. However, the specific role of LIN28B protein and its mechanism of action in MB had not been studied to date. This study assessed the role of LIN28B in Daoy MB cells using siRNA-mediated silencing of LIN28B. Supported by proteomics analysis and cell-based assays, silencing of LIN28B reduced the viability of MB cells, as well as ribosome biogenesis, and promotes cellular lipid accumulation. This study highlights the importance of LIN28B as a promising target to regulate MB cell growth, ribosomal biogenesis and lipid metabolism.