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Evaluation of the antimicrobial potential of indigenous probiotic strain of Lactobacillus plantarum against skin pathogens Staphylococcus aureus MTCC 96 and Propionibacterium acne MTCC 1950
  • Ashok Yadav
Ashok Yadav
Central University of Jammu

Corresponding Author:[email protected]

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Abstract

In this comprehensive study, the antimicrobial activity of selected probiotic L. plantarum isolates against prevalent skin pathogens Staphylococcus aureus MTCC 96 and Propionibacterium acne MTCC 1950 were evaluated. Our findings showcased a spectrum of antimicrobial efficacy among these isolates, ranging from 10 mm ± 0.71 to 20 mm ± 0.58 against S. aureus MTCC 96 and P. acne MTCC 1950. The study delved further into the nature of antimicrobial agents by subdividing conditioned cell-free supernatants (CFS) of L. plantarum isolates into three categories. Results indicated that while 15 isolates were positive against S. aureus, only two isolates (LpJ4 and LpJ5) maintained positivity with conditioned CFS treatment. Similarly, although fourteen isolates exhibited positive results against P. acne with non-conditioned CFS, only three isolates (LpJ1, LpJ15, and LpJ19) demonstrated zone of inhibition (>14mm) with conditioned CFS. These findings emphasized the heightened antimicrobial activity of non-conditioned CFS due to the presence of organic acids. Moreover, it suggested a potential role of proteins in this antimicrobial activity, as demonstrated by the loss of inhibitory effects following treatment with proteinase K. Furthermore, our study explored the auto-aggregation and co-aggregation capabilities of these probiotic isolates, indicating their potential to adhere to gastrointestinal epithelial cells and facilitate microorganism interactions. The understanding of these factors offers substantial potential for the development of precise therapies aimed at addressing skin infections attributed to these pathogens.