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Heterologous expression of P9 from Akkermansia muciniphila improves the GLP-1 secretion of intestinal L cells.
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  • Wenxuan Di,
  • Yuchen Zhang,
  • xinyuan zhang,
  • Luxuan Han,
  • Liang Zhao,
  • Yanling Hao,
  • Zhengyuan Zhai
Wenxuan Di
China Agricultural University
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Yuchen Zhang
China Agricultural University
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xinyuan zhang
Beijing University of Agriculture
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Luxuan Han
China Agricultural University
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Liang Zhao
China Agricultural University
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Yanling Hao
China Agricultural University
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Zhengyuan Zhai
China Agricultural University

Corresponding Author:[email protected]

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Abstract

Glucagon-like peptide-1(GLP-1) is an incretin hormone secreted primarily from the intestinal L-cells in response to meals. GLP-1 is a key regulator of energy metabolism and food intake. It has been proven that P9 protein from A. muciniphila could increase GLP-1 release and improve glucose homeostasis in HFD-induced mice. To obtain an engineered Lactococcus lactis which produced P9 protein´╝îmature polypeptide chain of P9 was codon-optimized, fused with N-terminal signal peptide Usp45, and expressed in L. lactis NZ9000. Heterologous secretion of P9 by recombinant L. lactis NZP9 were successfully detected by SDS-PAGE and western blotting. Notably, the supernatant of L. lactis NZP9 stimulated GLP-1 production of NCI-H716 cells. The relative expression level of GLP-1 biosynthesis gene GCG and PCSK1 were upregulated by 1.63 and 1.53 folds, respectively. The engineered strain L. lactis NZP9 was more effective than A. muciniphila BAA-835 in stimulating the intestinal L cells to produce GLP-1. To our knowledge, this is the first report on the secretory expression of carboxyl-terminal processing protease P9 from A. muciniphila in L. lactis. Our results suggest that genetically engineered L. lactis which expressed P9 may have therapeutic potential for the treatment of diabetes, obesity and other metabolic disorders.