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Engineering yeast lipids for production of designer biodiesel
  • Benjamin Ouellet,
  • Ahmad Abdel-Mawgoud
Benjamin Ouellet
Laval University Faculty of Science and Engineering
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Ahmad Abdel-Mawgoud
Laval University Faculty of Science and Engineering

Corresponding Author:[email protected]

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Biodiesels constitute a growing class of fuel in the world which is increasingly inclined towards more ecological and sustainable energy. Despite their advantages, biodiesels are of limited cold flow properties and of larger NOx emissions, which are mostly attributed to the chemical composition of their oil feedstocks. This study presents a novel approach to produce Genetically Engineered Biodiesel from genetically manipulated oleaginous yeast oils for improving biodiesel properties and performances. Using full-factorial central composite design, the best chemical composition of an optimal biodiesel was predicted. Then, simple and combined MFE1, PEX10 and POX2 mutants of the oleaginous yeast Yarrowia lipolytica were constructed and showed interesting lipid profiles whose biodiesel is predicted to have better cold flow properties. These mutants showed also higher lipid titers by 2-3 folds compared to the parent strain. This study provides a genetic engineering strategy for tailor design of biodiesel properties and performance.
27 Sep 2023Submitted to Biotechnology Journal
28 Sep 2023Assigned to Editor
28 Sep 2023Submission Checks Completed
29 Sep 2023Reviewer(s) Assigned
15 Oct 2023Review(s) Completed, Editorial Evaluation Pending
15 Oct 2023Editorial Decision: Revise Major