One of the most dangerous substances, 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), has a devastating lethal effect on both animal and human tissues. It is representative of numerous environmental pollutants known as halogenated polycyclic hydrocarbons. As a result, significant efforts are being made to reduce the damaging effects of TCDD. Additionally, boron compounds are employed in a variety of sectors, from agriculture to the production of cosmetics and medicines. They have an impact on crucial cellular processes and enzymatic activities. Boric acid (BA) and other boron compounds, such as ulexite (UX) and borax (BX), which are widely used commercially, have little information on dosage-related effects. In the current investigation, PBMCs were treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which caused cytotoxicity and damaged membrane integrity. It led to a decrease in antioxidant status and an increase in MDA levels. The treatment with TCDD also promoted genomic damage by raising the levels of 8-OH-dG and CA frequency. Furthermore, boron compounds dramatically reduced the cytotoxicity, genotoxicity and oxidative damage induced by TCDD. Our findings imply that, at certain concentrations, boron compounds may function as a possible chemopreventive against dioxin-induced cytotoxicity and genotoxicity in PBMCs.