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Classic Human Astrovirus 4, 8, MLB-3 and likely new genotype 5 sub-lineage in stool samples of children with Acute Flaccid Paralysis (AFP) in Nigeria
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  • Adewumi Moses Olubusuyi,
  • IJEOMA IFEORAH,
  • Faleye O. C. Temitope,
  • Lander De Coninck,
  • Agbaje Tunde Sheriff,
  • George Etop Uwem,
  • Onoja Ayenbe Bernard,
  • Olayinka Adebowale Oluseyi,
  • Oni Igbekele Elijah,
  • Ajileye Goodnews Toluwani,
  • Oragwa Obinna Arthur,
  • Akinleye Emmanuel Toluwanimi,
  • Popoola Olubukola Bolutife,
  • Osasona Gideon Damilola,
  • Olayinka Olaitan Titilola,
  • George Adefunke Oluwadamilola,
  • Ahmed Iluoreh Muhammad,
  • Komolafe Isaac Omotosho,
  • J. A. Adeniji,
  • Jelle Matthijnssens
Adewumi Moses Olubusuyi
University of Ibadan College of Medicine

Corresponding Author:[email protected]

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IJEOMA IFEORAH
University of Nigeria - Enugu Campus
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Faleye O. C. Temitope
Arizona State University Biodesign Center for Environmental Health Engineering
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Lander De Coninck
Katholieke Universiteit Leuven Rega Institute for Medical Research Laboratorium Virologie en Chemotherapie
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Agbaje Tunde Sheriff
University of Ibadan College of Medicine
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George Etop Uwem
Redeemer's University
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Onoja Ayenbe Bernard
University of Ibadan College of Medicine
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Olayinka Adebowale Oluseyi
University of Ibadan College of Medicine
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Oni Igbekele Elijah
University of Ibadan College of Medicine
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Ajileye Goodnews Toluwani
University of Ibadan College of Medicine
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Oragwa Obinna Arthur
University of Jos
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Akinleye Emmanuel Toluwanimi
University of Ibadan Faculty of Pharmacy
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Popoola Olubukola Bolutife
University of Ibadan College of Medicine
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Osasona Gideon Damilola
Redeemer's University
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Olayinka Olaitan Titilola
University of Ibadan College of Medicine
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George Adefunke Oluwadamilola
National Veterinary Research Institute
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Ahmed Iluoreh Muhammad
University of Ibadan College of Medicine
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Komolafe Isaac Omotosho
Redeemer's University Faculty of Natural Sciences
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J. A. Adeniji
University of Ibadan College of Medicine
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Jelle Matthijnssens
Katholieke Universiteit Leuven Rega Institute for Medical Research Laboratorium Virologie en Chemotherapie
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Abstract

Introduction: Acute viral gastroenteritis is a common illness especially in developing countries. Globally, the role of human astroviruses (HAstV) in gastroenteritis among children and elderly is well documented. But there exists a substantial dearth of information on HAstV strains circulating in Nigeria. Method: Viral like particles (VLPs) were purified from stool samples from children diagnosed with acute flaccid paralysis (AFP) between January and December 2020 from five states in Nigeria, using the NetoVIR protocol. Extracted viral RNA and DNA were subjected to a reverse transcription step, and subsequent random PCR amplification. Library preparation and Illumina sequencing were performed. Using the Virome Paired-End Reads (ViPER) pipeline, raw reads were processed into genomic contigs. Phylogenetic and pairwise identity analysis of the recovered HAstV genomes was performed. Results: Six near complete genome sequences of HAstV were identified and classified as HAstV 4 (n=1), HAstV5 (n=1), HAstV8 (n=1) and MLB-3 (n=3). The HAstV5 belonged to a yet unclassified sub-lineage which we tentatively named HAstV-5d. Phylogenetic analysis of ORFs 1a, 1b and 2 suggested recombination events inside the MAstV1 species. Furthermore, phylogenetic analysis implied a geographic linkage between the HAstV5 strain from this study with two strains from Cameroon across all the genomic regions. Conclusion: We report for the first time the circulation of HAstV genotypes 4, 8 and MLB-3 in Nigeria and present data suggestive for the existence of a new sub-lineage of HAstV5. To further understand the burden, diversity, and evolution of HAstV increased research interest as well as robust HAstV surveillance in Nigeria is essential.
30 Aug 2023Submitted to Journal of Medical Virology
30 Aug 2023Assigned to Editor
30 Aug 2023Submission Checks Completed
30 Aug 2023Review(s) Completed, Editorial Evaluation Pending
05 Sep 2023Reviewer(s) Assigned
01 Oct 2023Editorial Decision: Revise Major