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Improving the properties of platelet-rich fibrin (PRF) gel with tannic acid as a green crosslinker
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  • tahereh Haghparast-Kenarsari,
  • majid Abdouss,
  • saeed Heidari keshel,
  • Zoheir Heshmatipour,
  • azam Rahimi ,
  • Esmaeil Biazar
tahereh Haghparast-Kenarsari
Islamic Azad University Central Tehran Branch
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majid Abdouss
Amirkabir University of Technology
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saeed Heidari keshel
Shahid Beheshti University
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Zoheir Heshmatipour
Islamic Azad University Tonekabon Branch
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azam Rahimi
Shahid Beheshti University of Medical Sciences
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Esmaeil Biazar
Islamic Azad University Tonekabon Branch

Corresponding Author:[email protected]

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Platelet-rich fibrin (PRF), which is the rich source of growth factors, has been used as an efficient scaffold in tissue regeneration. In this study, tannic acid as a green crosslinker with different concentrations (0.5%,1%,5%, and 10%) was used to improve the properties of PRF. The crosslinked gel scaffolds were evaluated by analyses such as SEM, FTIR, swelling and degradation, mechanical strength, cell toxicity, cell adhesion, and antibacterial test. The results showed that the scaffold structure changes by increasing crosslinker concentration. The swelling rate decreased from 49% to 5% for samples without the crosslinker and with tannic acid (10%), respectively. A decrease in the degradation rate was shown by increasing the crosslinker concentration. The mechanical strength of the scaffold with the crosslinker increased up to three times. Cytotoxicity was not observed up to 10% crosslinker concentration. The cells proliferated well on the cross-linked scaffolds and also showed a good antibacterial effect. In general, tannic acid can improve the physical and mechanical properties of PRF without negatively affecting its biological properties.