Purification of Functional Soybean Oligosaccharides using Yeast PL08 by
In order to provide a theoretical premise for the purification of soy
oligosaccharides from soybean whey, this paper examines the yeast PL08
fermentation process for purifying functional soybean oligosaccharides.
Using the logistic model and the Luedeking-Piret model, the kinetics of
PL08 cell growth, sucrose consumption, and ethanol production were
determined by nonlinear data fitting. The linearity of the models was as
high as 0.98, indicating the viability of this method. At 30h, the batch
fermentation procedure revealed that the purity of functional
oligosaccharides was 94.12%, while the ethanol concentration continued
to rise to 3.0%vol. Based on the results of kinetic studies, a 10L
exponential feeding culture was conducted, and the results indicated
that the alcohol concentration can be controlled below 0.10%vol, the
maximum biomass concentration was 50.01g/L, and the maximum purity of
functional soybean oligosaccharides was 95.59% in 21h.