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Chalcones accelerate the recovery period after cuprizone-induced noxious changes in the C57BL/6 mice model
  • Soodeh Rowhanirad,
  • Mahnaz Taherianfard
Soodeh Rowhanirad
Shiraz University

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Mahnaz Taherianfard
School of vet med
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Abstract

Multiple sclerosis (MS) is the most common demyelinating disorders of the CNS. Despite advancements in medicine, there remains an absence of an efficient cure without side effects and MS patients suffer from the progressive symptoms. On the other hand, the impact of natural compounds and the mechanisms of their actions in ameliorating neurodegenerative disorders have been poorly known. The current study investigated the effect of Chalcones from Ashitaba (ChA) on accelerating the recovery period in the Cuprizone model. The control group with no Cuprizone or ChA treatment (CNT), Cuprizone-exposed 1 and 2 (CPZ1, CPZ2) groups with 0.2% Cuprizone feeding for five weeks without and with recovery period; respectively. And ChA-treated (Treated1, Treated2) groups received ChA at 300 or 600 mg/kg/day; respectively, for 2 weeks following cuprizone removal. The degree of demyelination in the corpus callosum (CC), impairment of cognitive function, and serum and brain levels of Brain-Derived Neurotrophic Factor (BDNF) and Tumor Necrosis Factor Alpha (TNFα) were evaluated by the histological, Y-maze, and enzyme-linked immunosorbent assay (ELISA) tests, respectively. The results demonstrated that the ChA treatment significantly improved the serum and brain levels of BDNF and the behavioral responses compared to the CPZ-exposed groups. Moreover, the ChA-treated groups had a significant decrease in the serum and brain TNFα levels and the extent of CC demyelination compared to the CPZ-exposed groups. The present study showed that ChA treatment for 2 weeks accelerated remyelination of the CC through suppressing inflammation caused by TNFα and promoting myelination resulted from BDNF.