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Ferritin-Nanocaged Aggregation-Induced Emission Nanoaggregates for NIR-II Fluorescence-Guided Noninvasive, Controllable Male Contraception
  • +11
  • Xinghua Yu,
  • Jiaxue Shuai,
  • Ge Meng,
  • Shumin Zhou,
  • Amali Wijayaraja,
  • Yixiang Zhao,
  • Lei Yao,
  • Rui Yao,
  • Xing Yang,
  • Tianfu Zhang,
  • Liying Wang,
  • Pengyu Gu,
  • Pengfei Zhang,
  • Fei Sun
Xinghua Yu
Zhejiang University School of Medicine

Corresponding Author:[email protected]

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Jiaxue Shuai
Zhejiang University School of Medicine
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Ge Meng
Zhejiang University School of Medicine
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Shumin Zhou
Zhejiang University School of Medicine
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Amali Wijayaraja
Zhejiang University School of Medicine
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Yixiang Zhao
Zhejiang University School of Medicine
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Lei Yao
Zhejiang University School of Medicine
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Rui Yao
Zhejiang University School of Medicine
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Xing Yang
Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences
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Tianfu Zhang
Guangzhou Medical University
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Liying Wang
Zhejiang University School of Medicine
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Pengyu Gu
Zhejiang University School of Medicine
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Pengfei Zhang
Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences
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Fei Sun
Zhejiang University School of Medicine
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Abstract

Controllable contraception in male animals was demonstrated through the utilization of gold nanorods’ photothermal effect to accomplish mild testicular hyperthermia. However, the challenges arising from testicular administration and the non-biodegradability of nanoparticles hinder further clinical implementation. Therefore, a straightforward, non-invasive, and enhanced contraception approach is required. This study explores the utilization of human heavy chain ferritin (HFn) nanocarriers loaded with aggregation-induced emission luminogens (AIEgens) for noninvasive, controllable male contraception guided by Near-Infrared-II (NIR-II) fluorescence imaging. The HFn-caged AIEgens (HFn@BBT) are delivered via intravenous injection and activated by near-infrared irradiation. Lower hyperthermia treatment induces partial damage to the testes and seminiferous tubules, reducing fertility indices by approximately 100% on the 7th day, which gradually recovers to 80% on the 60th day. Conversely, implementation of elevated hyperthermia therapy causes total destruction of both testes and seminiferous tubules, leading to a complete loss of fertility on the 60th day. Additionally, the use of AIEgens in NIR-II imaging offers improved fluorescence efficiency and penetration depth. The findings of this study hold significant promise for the advancement of safe and effective male contraceptive methods, addressing the need for noninvasive and controllable approaches to reproductive health and population control.