loading page

Advances in common in vitro cellular models of pulmonary fibrosis
  • +4
  • Die Li,
  • Weibin Qian,
  • Xinyue Zhang,
  • Ziqiong Song,
  • Shan Zhao,
  • Yuan Huang,
  • Xinrui Cai
Weibin Qian

Corresponding Author:[email protected]

Author Profile
Xinyue Zhang
Author Profile
Ziqiong Song
Author Profile
Yuan Huang
Author Profile
Xinrui Cai
Author Profile


The establishment of in vitro models plays a vital role in understanding and investigating pulmonary fibrosis (PF) at the cellular and molecular levels. In this paper, we conduct a literature review and provide an analysis of various cellular models used in scientific experiments, along with their applications in understanding the pathogenesis of PF. Our studies indicate that a comprehensive understanding of PF should not be based on a single cell type or organ, but on a multi-organ, multi-level, and multi-perspective approach. Primary cells demonstrate superior cell growth characteristics and gene expression profiles. However, challenges such as limited availability, difficulties in maintenance, inability for continuous propagation, and susceptibility to phenotype loss over time significantly restrict their utility in scientific research. On the other hand, replacement cell lines can be easily obtained, cultured, and continuously propagated, but their phenotypic characteristics are somewhat different compared to primary cells. In vitro co-culture models offer a more practical and precise means to elucidate the intricate interactions between cells, tissues, and organs. Therefore, when constructing pathology models of PF, researchers should carefully consider the advantages, limitations, and relevant mechanisms associated with different cell models for selection according to the research objectives.
12 Aug 2023Submitted to Immunology & Cell Biology
13 Aug 2023Assigned to Editor
13 Aug 2023Submission Checks Completed
13 Aug 2023Reviewer(s) Assigned
01 Nov 2023Review(s) Completed, Editorial Evaluation Pending
14 Nov 2023Editorial Decision: Revise Major