loading page

Sericin improve diabetic cognitive impairment in rats by inhibiting TXNIP/NLRP3 neuroinflammation through SIRT1
  • +15
  • Pingjing Wen,
  • Dan Yi,
  • Guangqiu Qin,
  • Jinyue Li,
  • Yuqiu Gao,
  • Haichen Cui,
  • Yixin Ye,
  • Ziheng Xu,
  • Haixin Liu,
  • Liping Wu,
  • Xianjin Lei,
  • Yue Wu,
  • Pei Qin,
  • Yiyi Ling,
  • Yuanzhao Zhou,
  • Yuyi Chen,
  • Jiaheng Du,
  • Baiqing Dong
Pingjing Wen
GuangXi University of Chinese Medicine

Corresponding Author:[email protected]

Author Profile
Dan Yi
GuangXi University of Chinese Medicine
Author Profile
Guangqiu Qin
GuangXi University of Chinese Medicine
Author Profile
Jinyue Li
GuangXi University of Chinese Medicine
Author Profile
Yuqiu Gao
GuangXi University of Chinese Medicine
Author Profile
Haichen Cui
GuangXi University of Chinese Medicine
Author Profile
Yixin Ye
GuangXi University of Chinese Medicine
Author Profile
Ziheng Xu
GuangXi University of Chinese Medicine
Author Profile
Haixin Liu
GuangXi University of Chinese Medicine
Author Profile
Liping Wu
GuangXi University of Chinese Medicine
Author Profile
Xianjin Lei
GuangXi University of Chinese Medicine
Author Profile
Yue Wu
GuangXi University of Chinese Medicine
Author Profile
Pei Qin
GuangXi University of Chinese Medicine
Author Profile
Yiyi Ling
GuangXi University of Chinese Medicine
Author Profile
Yuanzhao Zhou
GuangXi University of Chinese Medicine
Author Profile
Yuyi Chen
GuangXi University of Chinese Medicine
Author Profile
Jiaheng Du
GuangXi University of Chinese Medicine
Author Profile
Baiqing Dong
GuangXi University of Chinese Medicine
Author Profile

Abstract

Aims: The aim of this study was to examine the effects of sericin on diabetic cognitive impairment (DCI) in rats based on neuroinflammation. Methods: SD rats were firstly fed with high sugar and high fat diet for 4 weeks, and then injected with 50 mg/kg streptozotocin intraperitoneally to establish a diabetic model. The diabetic rats were randomly divided into 3 groups and treated with distilled water (n=10), 500 mg/kg (n =10) and 1000 mg/kg (n=20) sericin, respectively, by gavage once a day for 8 weeks. Before the end of the trail, 10 rats in the 1000 mg/kg sericin group were injected with 10 μg EX527 (a SIRT1 inhibitor) into the lateral ventricles once every other day for 5 times. Results: Treated with sericin significantly reduced the fasting blood glucose, improved DCI in rats. Sericin significantly inhibited of neuroinflammation, reduced the expression of NLRP3, TXNIP proteins and reduced cell apoptosis, while increased the expression of SIRT1 protein in the hippocampus of diabetic rats. After inhibiting SIRT1 with EX527, the above effect of sericin on DCI rats was weakened. Conclusions: These results indicated that sericin may block DCI progression in rats by inhibiting TXNIP/NLRP3 neuroinflammation and neuronal apoptosis though SIRT1.