Establishment of a novel obesity mouse model: The induction of
intestinal microbiota dysbiosis
Objective To establish and evaluate an intestinal microbiota
dysbiosis-induced obesity mouse model. Methods 50 C57BL/6J male healthy
mice were randomly divided into an obesity model group and the control
group. The body weight, body length, and Lee’s index of the two groups
of mice at week 1 and week 10 were compared. Serum glucose (GLU), total
cholesterol (TC) and triglyceride (TG) were measured by enzyme-labeled
colorimetric methods. Illumina HiSeq 16S rDNA high-throughput sequencing
technology was used to characterize intestinal microbiota in feces.
Results The success rate of model establishment in obese mice was 52%.
The body weight, body length, Lee’s index, and abdominal fat (wet
weight) in the obese model group were all higher than those in the
control group, and the differences were statistically significant
(P＜0.01). Serum GLU and TC levels in the obesity model group were
higher than those in the control group (P＜0.05) , and there was no
difference in TG levels between the two groups（P>0.05）.
The control group contained more abundant intestinal microbiota phylum
and genera than did the obesity model group; the differences between the
two groups were significant（FDR≤0.05，P≤0.05）. Conclusion Intestinal
microbiota dysbiosis can be used to generate an obesity model in mice.