Comparative proteome analysis of retinal hypoxia--ischemia in acute
ocular hypertension model with TMT-based quantitative proteomics
Acute glaucoma’s main sign is acute ocular hypertension (AOH), leading
to retinal ganglion cell (RGC) death and irreversible visual loss.
However, there is currently no approved effective therapy for this
condition. This research aimed to identify the major regulators and the
overall protein changes involved in AOH-induced RGC death.
Mass-spectrometry was used to analyze proteomic patterns in the retinal
protein extracts from the AOH and sham-group, and then Gene Ontology
(GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway studies
were performed. In proteomics analysis, we identified 92 proteins in the
AOH group compared to the control group, with 58 proteins being
up-regulated and 34 proteins being down-regulated. Western blot and
biochemical assay analyses identified changes in Fatty acid-binding
protein 7 (FABP7), and caveolin-1(Cav-1) that were related to fatty acid
metabolism and ocular inflammatory signaling. Moreover, variations in
the expression of the proteins Galectin-1 (Gal-1), S100 calcium-binding
protein A6 (S100a6), and Visinin-like protein-1 (VILIP) was shown, all
of which were associated to the process of neuronal ischemia. Our
investigation demonstrated that neuroinflammation and fatty acid
metabolism were involved in retinal impairment following AOH, offering a
potential therapeutic strategy for acute glaucoma.