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MORF9 mediated plastid RNA editing influences Arabidopsis root growth under sugar starvation
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  • Ying Miao,
  • Yakun Xie,
  • Jinfa Yu,
  • Faan Tian,
  • Xue Li,
  • Xinyan Chen,
  • Yanyun Li,
  • Binghua Wu
Ying Miao
Fujian Agriculture and Forestry University

Corresponding Author:[email protected]

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Yakun Xie
Fujian Agriculture and Forestry University
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Jinfa Yu
Fujian Agriculture and Forestry University
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Faan Tian
Fujian Agriculture and Forestry University
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Xue Li
Fujian Agriculture and Forestry University
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Xinyan Chen
Fujian Agriculture and Forestry University
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Yanyun Li
Fujian Agriculture and Forestry University
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Binghua Wu
Fujian Agriculture and Forestry University
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Abstract

RNA editing is a tightly controlled process by which cytidines are converted to uridines in RNAs transcribed from the chloroplast and mitochondrial genomes in flowering plants. Multiple organellar RNA editing factor (MORF) complex was recently shown to be highly associated with C-to-U RNA editing activity of vascular plant editosome. However, mechanisms by which MORF9 mediates plastid RNA editing to control plant development in response to environmental cues remains obscure. In this study, we found that loss of MORF9 function impaired PSII efficiency, NDH activity, and carbohydrate production, rapidly promoted nuclear gene expression including sucrose transporter and sugar/energy responsive genes, and attenuated seedling development under sugar starvation conditions. Sugar repletion increased MORF9 and MORF2 expression in wild-type seedlings and promoted inefficiency of matK-706C, accD-794C, ndhD-383C and ndhF-290C RNA editing in morf9 mutant. This RNA editing inefficiency was associated with altered cell division in root meristem zone and nuclear gene expression in the morf9 mutant. Using gin2, snrk1, morf9 single and double mutants and overexpression of SnRK1 (KIN10) or HXK1 in the morf9 mutant background demonstrated that RNA editing efficiency of ndhD-383C and ndhF-290C sites was diminished in the gin2/morf9 double mutants, and editing efficiency of matK-706C, accD-794C, ndhD-383C and ndhF-290C sites was significantly diminished in the snrk1/morf9 double mutants. Overexpressing HXK1 or SnRK1 promoted RNA editing rate of matK, accD, ndhD, and ndhF in leaves of morf9 mutants´╝îindicating that HXK1 might be required for MORF9 mediated ndhD-383C and ndhF-290C editing, while SnRK1 may only be required for MORF9 mediated ndhF-290C site editing. Collectively these findings suggest that sugar and/or its intermediary metabolites impair MORF9 mediated plastid RNA editing resulting in derangements of plant root development.
04 Aug 2023Submitted to Plant, Cell & Environment
04 Aug 2023Submission Checks Completed
04 Aug 2023Assigned to Editor
04 Aug 2023Review(s) Completed, Editorial Evaluation Pending
14 Aug 2023Reviewer(s) Assigned
30 Oct 2023Editorial Decision: Revise Minor
24 Jan 20242nd Revision Received
24 Jan 2024Submission Checks Completed
24 Jan 2024Assigned to Editor
27 Jan 2024Review(s) Completed, Editorial Evaluation Pending
01 Feb 2024Editorial Decision: Accept