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Enterococcus faecium Bacteriocin Efflux Pump MexA Gene and promote skin wound healing in mice
  • mais ahmed
mais ahmed
University of Baghdad

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Abstract

Isolation of E. faecium from honeybee gut and screening of bacteriocin production from this bacterium and assessing its effects on MexA gene expression of multidrug resistant (MDR) P. aeruginosa and assessing the role of bacteriocin in treating infected wound in mice by histopathological examination.The results showed a significant inhibition zone of 20mm. The optimum conditions for Bacterocin production evaluated found that yeast extract was the most suitable nitrogen source and glucose was a better carbon source. The optimal incubation period was 72 hours and pH was determined to be 5. Another aspect of the study focused on identifying MDR P. aeruginosa isolates from wound infections. The identification was carried out using VITEK 2 system, The results of antibiotics susceptibility tests revealed that the greatest resistance rates was found against Meropenem (83.3%) and Gentamicin (73.3%), followed by beta lactam antibiotics (Ticarcillin, Ticarcillin/Clavulanic Acid, Piperacillin and Aztreonam) showed resistance in about 66.6 and 36.6% of the study isolates. Followed by Imipenem 63.3%, Ceftazidime 36.6% and Cefepime 36.6%. The MexA gene was detected in all nine strains. The study also investigated the impact of the Bacterocin of the chosen strain on the expression of the MexA gene. . In vivo study revealed that wound healing was enhanced by treated infected wound with E. faecium bacteriocin. Conclusion: Down-regulation and up-regulation in the expression of the genes following exposure to Bacterocin, indicating the potential of E. faecium as an effective antimicrobial agent against MDR P. aeruginosa infections.