loading page

Extrahepatic beta cell transplantation supported by three dimensional nanofiber scaffold for the treatment of diabetes mellitus
  • Arun Koyyada,
  • Prabhakar Orsu
Arun Koyyada
GITAM (Deemed to be University)

Corresponding Author:[email protected]

Author Profile
Prabhakar Orsu
GITAM (Deemed to be University)
Author Profile

Abstract

Transplantation of islets with supporting three dimensional (3D) scaffolds could be an effective treatment modality in diabetes by overcoming meagre survivability and stunted proliferation of cells associated with direct islets transplantation. We have transplanted RIN-5F (rat insulinoma) cells seeded nanofiber scaffold (carboxymethyl guargum + reduced graphene oxide) in diabetic rats at epididymal fat pad site. The porous architecture of this scaffold has improved cell infiltrations and aid in the neovascularization of beta cells. The implanted scaffold rendered normoglycemia (blood glucose <200 mg/dl) in rats within six days compared to direct RIN-5F cells (without scaffold) transplanted group. The histology of the newly developed graft in the scaffold group demonstrated neovascularisation, fibrinogen and collagen production. RT-PCR results showed upregulated Nkx 6.1 (transcription factor) and VEGFa gene expression (vascular endothelial growth factor). Therefore, this polymer scaffold could act as a potential platform for in vivo transplantation of beta cells in treating diabetes