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Fish Mucus Stabilized Biosynthesis of Zinc Oxide Nanoparticles and their Structural Properties: Evaluation in vitro anticancer efficiency against human cervical cancer cells (HeLa)
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  • Ramasamy Subramanian,
  • Govindasamy Chinnadurai,
  • Raman Suresh,
  • Navaneethan Renuka,
  • Govindasami Periyasami,
  • Perumal Karthikeyan
Ramasamy Subramanian
Sun Arts and Science College

Corresponding Author:[email protected]

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Govindasamy Chinnadurai
Sacred Heart Arts & Science College
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Raman Suresh
Government Polytechnic College
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Navaneethan Renuka
Abu dhabi Men’s College Higher College of Technology
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Govindasami Periyasami
King Saud University Geology and Geophysics Department
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Perumal Karthikeyan
The Ohio State University Department of Chemistry and Biochemistry
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Abstract

In the present work, the epidermal mucus of Channa striatus ( C.striatus) stabilized the biosynthesis of zinc oxide nanoparticles (ZnO NPs) were synthesized. The nanoparticles were characterized using an Ultra-visible spectrophotometer (UV-Vis), Fourier transform infrared spectrophotometer (FT-IR), X-ray diffraction (XRD), and scanning electron microscope coupled with EDS. Various shapes like rod, hexagonal, A high-resolution transmission electron microscope (HR-TEM) reveal s the shape and selected area diffraction pattern. The samples were screened for toxicity using human cervical cancer cells (HeLa). The XRD pattern of mucus-stabilized ZnO NPs exhibits smaller size than that of the ZnO NPs without mucus. The anticancer efficiency of the ZnO NPs was examined by MTT assays in HeLa cells at different doses. ZnO NPs synthesized with 1 and 2 mL mucus exhibit 27.40 and 18.84 % of cell viability at 100 µg/ml. The microscopy results recommend that the synthesized ZnO NPs controlled the growth of the HeLa cells effectively at the concentration of 100 µg/mL. Therefore, mucus stabilized ZnO NPs can be used as an anticancer agents for the treatment of HeLa cells.