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Processing stallion semen for on farm use and cooled transport
  • Dale Kelley
Dale Kelley
Texas A&M University College Station

Corresponding Author:[email protected]

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Simple dilution of semen is the oldest method to process semen for artificial insemination with either fresh or cooled. This was developed prior to the optimization centrifugation techniques for semen through a combination of trial and error and research. Simple dilution and is a faster method to process semen, is easy to use and works in many circumstances. For on farm semen use a 1:1 (v/v) semen to extender ratio is used. A variety of other techniques have been developed, such as cushion centrifugation, density gradient centrifugation and sperm filtration. Cushion centrifugation is used to concentrate the sperm and allows higher centrifugation speeds than centrifugation alone. This allows for improved recovery rates of sperm. Density gradient centrifugation has been used to select for morphologically normal sperm but suffers from low recovery rates. Processing sperm with this technique can result in improved motility and improved DNA integrity of sperm. Sperm filtration is another method to concentrate sperm without the use of centrifugation; however, this technique has lower recovery rates compared to cushion centrifugation. This review will discuss these techniques in the application of processing equine semen for either fresh or cooled-shipped use.
26 Jul 2023Submitted to Equine Veterinary Education
26 Jul 2023Submission Checks Completed
26 Jul 2023Assigned to Editor
27 Jul 2023Reviewer(s) Assigned
13 Aug 2023Review(s) Completed, Editorial Evaluation Pending
15 Aug 2023Editorial Decision: Revise Minor
15 Aug 20231st Revision Received
16 Aug 2023Assigned to Editor
16 Aug 2023Submission Checks Completed
17 Aug 2023Editorial Decision: Revise Minor
17 Aug 20232nd Revision Received
18 Aug 2023Submission Checks Completed
18 Aug 2023Assigned to Editor
22 Aug 2023Editorial Decision: Accept