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Transcription needs translation initiation of the downstream gene to continue downstream at intercistronic junctions in E. coli
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  • Heung Jin Jeon,
  • Monford Paul Abishek N,
  • Yonho Lee,
  • Jeongok Park,
  • Heon Lim
Heung Jin Jeon
Chungnam National University

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Monford Paul Abishek N
Chungnam National University
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Yonho Lee
Chungnam National University
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Jeongok Park
Chungnam National University
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Heon Lim
Chungnam National University
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Abstract

We have reported a gal mutant called galE stop0, wherein the galE stop codon was changed to a sense codon. The experiment results demonstrated that preventing galE translation termination inhibited the production of galE 3’ ends. This implies that when the galE translation termination was prevented, the galE 3’ ends generation was reduced or impaired. We anticipated that the translation of galE would continue to galT, producing a chimeric protein GalE-GalT. This study verified that the chimeric protein was produced, but unexpectedly, we found that the GalT protein was also synthesized in the mutant, and its amount equaled that in the wild-type. In the wild-type, we also found that the GalE-GalT chimeric protein was produced in an amount equal to that of the GalE protein. These results suggest that translation termination of galE and translation initiation of galT occur independently, thus, corroborating our transcription-translation model: At the cistron junction, transcription, decoupled from translation due to the translation termination of galE, needs translation initiation of galT to continue downstream; otherwise, transcription would be terminated by Rho.