Analysis of Differentially Expressed Proteins in Lymph Fluids Related to
Lymphatic Metastasis in Breast Cancer Rabbit Model Guided by
Abstract: Objective: To identify differentially expressed proteins in
the lymph fluid of rabbits with breast cancer lymphatic metastasis and
normal rabbits, and to analyze and verify using proteomics technology.
Materials and Methods: Rabbits with breast cancer lymph node metastasis
and healthy rabbits were selected. Lymph fluids were precisely extracted
under the guidance of percutaneous contrast-enhanced ultrasound (CEUS).
Label-free quantitative proteomics (LFQ) was used to detect and compare
differences, and differential protein expression was obtained.
Bioinformatics analysis was performed using KEGG and GO analysis
software, selecting the most significantly differentially expressed
proteins. Finally, parallel reaction monitoring technology (PRM) was
applied for validation. Results: A total of 547 significantly
differentially expressed proteins were found in this study, including
371 upregulated proteins and 176 downregulated proteins. They were
mainly involved in various cellular and metabolic pathways, including
upregulated proteins such as BLVRA, IDH2, and downregulated proteins
such as PDXK. The upregulated proteins PDIA3, PRKAR1A, and ABCC4
participated in immune regulation, endocrine regulation, and anti-tumor
drug resistance regulation, respectively. Conclusion: These proteins are
involved in the pathophysiological processes of tumor development and
metastasis, and can be used as predictive indicators for breast cancer
metastasis and as new targets for treatment.