loading page

Canola meal valorization via hydrolysis for amino acid generation
  • Janitha Wanasundara,
  • Takuji Tanaka,
  • Sumudu Warnakulasuriya
Janitha Wanasundara
Agriculture and Agri-Food Canada

Corresponding Author:[email protected]

Author Profile
Takuji Tanaka
University of Saskatchewan College of Agriculture and Bioresources
Author Profile
Sumudu Warnakulasuriya
University of Saskatchewan College of Agriculture and Bioresources
Author Profile


This study investigated an alternative approach to valorizing canola proteins by hydrolyzing them to generate amino acids (AAs). Pre-treatment of cold-pressed (CP) cake and desolventized-toasted (DT) meal with ethanol (99%, v/v) followed by protein separation was studied as process optimizations to maximize protein recovery with higher purity. The optimum ethanol pre-treatment conditions to achieve a meal containing less than 1% oil was reached at a meal-to-ethanol ratio of 1:4 (w:w) and 50°C for 30 min extraction. The protein recovery reached the maximum at pH 12 and a meal-to-solvent ratio of 1:10 (w:v), yielding 73% and 33% recovery from ethanol pre-treated CP and DT meals, respectively, in a single extraction. Untreated and ethanol pre-treated meals were hydrolyzed with 6 M HCl (protein-to-acid ratio of 5 mg:2 mL) for 24 h at 110°C. The ethanol pre-treatment improved AA recovery and released 373 mg AA/g dry CP meal biomass (dbm) compared to 279 mg AA/g untreated CP cake dbm. However, no improvement in AA recovery upon ethanol pre-treatment of DT meal. H2SO4 was examined as an alternative acid. More than 80% of the total AAs of CP proteins were released with 3 M H2SO4, while for DT meal proteins, a 5 M concentration was needed to achieve the same. Commercial canola meals can be utilized for generating free AAs; however, the meal processing history may affect the yield.
06 Jun 2023Submitted to Journal of the American Oil Chemists' Society
08 Jun 2023Submission Checks Completed
08 Jun 2023Assigned to Editor
08 Jun 2023Review(s) Completed, Editorial Evaluation Pending
08 Jun 2023Reviewer(s) Assigned
26 Jun 2023Editorial Decision: Revise Minor
22 Jul 20231st Revision Received
24 Jul 2023Submission Checks Completed
24 Jul 2023Assigned to Editor
24 Jul 2023Review(s) Completed, Editorial Evaluation Pending
27 Jul 2023Reviewer(s) Assigned
11 Aug 2023Editorial Decision: Accept