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AsHSP26.2, a creeping bentgrass chloroplast small heat shock protein positively regulates plant development
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  • Hong Luo,
  • Chang Liu,
  • Kangting Dong,
  • Hui Du,
  • Xiaodong Wang,
  • Xin Li,
  • Ning Huang,
  • Qian Hu,
  • Xinbo Sun
Hong Luo
Clemson University Department of Genetics and Biochemistry

Corresponding Author:[email protected]

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Chang Liu
Hebei Agricultural University
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Kangting Dong
Hebei Agricultural University
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Hui Du
Hebei Agricultural University
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Xiaodong Wang
Hebei Agricultural University
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Xin Li
Hebei Agricultural University
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Ning Huang
Hebei Agricultural University
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Qian Hu
Clemson University Department of Genetics and Biochemistry
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Xinbo Sun
Hebei Agricultural University
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Abstract

Small heat shock proteins (sHSPs), a family of proteins with high level of diversity, significantly influence plant stress tolerance and plant development. We have cloned a creeping bentgrass chloroplast-localized sHSP gene, AsHSP26.2 responsive to IAA, GA and 6-BA stimulation. Transgenic creeping bentgrass overexpressing AsHSP26.2 exhibited significantly enhanced plant growth with increased stolon number and length as well as enlarged leaf blade width and leaf sheath diameters, but inhibited leaf trichomes initiation and development in the under-epidermis. Furthermore, AsHSP26.2 overexpression altered phytohormone biosynthesis and signaling transduction, resulting in elevated auxin and gibberellins (GA) accumulation. The results obtained provide novel insights implicating the sHSPs in plant growth and development regulation, and strongly suggest AsHSP26.2 to be a novel candidate for use in crop genetic engineering for enhanced plant biomass production and grain yield.