Comparison efficiency of Isotope Ratio Mass Spectrometry Coupled to
Liquid Chromatography Method and the Conventional Methods to Identify
Honey Adulteration, Iran
Rationale Over the years, many techniques have been developed
for honey adulteration detection. The several methods based on the AOAC
have been admitted to the in-house method confirmed by Iranian Standard
Organization (ISIRI). Besides the unprecedented fraud in honey, the
scientific requirements to apply high-tech procedures. Therefore,
unraveling these discrepancies requires comparisons between the elderly
and novel methods. Methods The conventional methods determined
different parameters such as reducing sugars before hydrolysis, sucrose
amount, fructose to glucose ratio, Proline, and hydroxy methyl furfural.
Applying novel IRMS technique determined δ13C values for glucose with
high quality and quantity. The honey carbohydrates, such as glucose,
fructose, disaccharide, and trisaccharide, were assessed using LC/IRMS.
Results Based on these findings regarding the conventional and
IRMS methods for testing samples. According to our results, the
conventional method successfully passed 18 to 20 samples by ISIRI
standards. However, the IRMS method showed different results, with only
2 out of 20 samples being original and pure without adulteration.
Conclusion We collected twenty samples from various regions and
examined them using two methods to determine their authenticity. The
first method used is the C13 isotope and isotope ratio mass spectrometry
(IRMS), while the second method is based on conventional methods using
ISIRI. After analyzing the results with Prism software, we found a
statistically significant difference between the confidence level of the
IRMS method and conventional methods, with a p-value less than 0.05.