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Methods for the relative quantitation of the human plasma lipidome by liquid chromatography coupled with mass spectrometry using minimal sample manipulation
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  • Diane Taylor,
  • Bebiana C. Sousa,
  • Greg West,
  • Aveline Neo Huipeng,
  • Andrea F. Lopez-Clavijo
Diane Taylor
Babraham Institute
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Bebiana C. Sousa
Babraham Institute
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Greg West
Babraham Institute
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Aveline Neo Huipeng
Babraham Institute
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Andrea F. Lopez-Clavijo
Babraham Institute

Corresponding Author:[email protected]

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Extraction protocols and liquid chromatography coupled with mass spectrometry (LC-MS) and tandem mass spectrometry methods (LC-MS/MS) for the measurement of five lipid categories, namely; glycerophospholipids, glycerolipids, sphingolipids, sterol lipids and fatty acyls in human plasma are described here. Step-by-step instructions are provided for the liquid/liquid extraction methods, including solution preparation and the non-endogenous lipid internal standards used. All instrumental conditions, chromatography columns and solutions required for the LC-MS and LC-MS/MS methods are also detailed.
26 Apr 2023Submitted to Rapid Communications in Mass Spectrometry
27 Apr 2023Submission Checks Completed
27 Apr 2023Assigned to Editor
27 Apr 2023Review(s) Completed, Editorial Evaluation Pending
11 May 2023Reviewer(s) Assigned
31 May 2023Editorial Decision: Revise Major
24 Jul 20231st Revision Received
25 Jul 2023Submission Checks Completed
25 Jul 2023Assigned to Editor
25 Jul 2023Review(s) Completed, Editorial Evaluation Pending
31 Jul 2023Reviewer(s) Assigned
29 Aug 2023Editorial Decision: Accept