loading page

Importance of biofilm formation for promoting plant growth under salt stress in Pseudomonas putida KT2440
  • +1
  • Stefanie Costa Gutierrez,
  • Enzo Raimondo,
  • Paula Vincent,
  • Ricardo de Cristóbal
Stefanie Costa Gutierrez
Instituto Superior de Investigaciones Biológicas

Corresponding Author:[email protected]

Author Profile
Enzo Raimondo
Planta Piloto de Procesos Industriales Microbiológicos
Author Profile
Paula Vincent
Instituto Superior de Investigaciones Biológicas
Author Profile
Ricardo de Cristóbal
Instituto Superior de Investigaciones Biológicas
Author Profile


An underutilized experimental design was used to isolate adapted mutants of the model bacterium Pseudomonas putida KT2440. The experimental design consisted of subjecting a random pool of mini-Tn5 mutants of P. putida KT2440 to several rounds of selection in the rhizosphere of soybean irrigated with NaCl solution. Isolated adapted mutants (MutAd) showed a mutation in a gene encoding the membrane-binding protein LapA, which is involved in the early stages of biofilm formation on abiotic surfaces. Two MutAd bacteria (MutAd160 and MutAd185) and a lapA deletion mutant were tested to study the effect of this gene on salt tolerance, rhizosphere fitness, extracellular polymeric substances (EPS) production, and plant growth promotion. The inability of the mutants to form biofilm did not hinder attachment to soybean seeds and roots. MutAd bacteria showed an overproduction of EPS when grown under saline conditions, which would compensate for the lack of biofilm formation. MutAd185 bacteria showed increased root attachment and growth promotion of soybean in slightly saline soils. The proposed experimental design would be useful to accelerate bacterial adaptation to the rhizosphere of plants under a given environmental condition, identify genetic mutations that benefit bacterial fitness in that condition, and thus increase their ability to promote plant growth.
18 Apr 2023Submitted to Journal of Basic Microbiology
19 Apr 2023Submission Checks Completed
19 Apr 2023Assigned to Editor
19 Apr 2023Review(s) Completed, Editorial Evaluation Pending
21 Apr 2023Reviewer(s) Assigned
13 May 2023Editorial Decision: Revise Major
20 Jun 20231st Revision Received
22 Jun 2023Submission Checks Completed
22 Jun 2023Assigned to Editor
22 Jun 2023Review(s) Completed, Editorial Evaluation Pending
24 Jun 2023Reviewer(s) Assigned
13 Jul 2023Editorial Decision: Accept